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LIG4 (ligase IV, DNA, ATP-dependent) Antibody | Aviva Systems Biology antibody product information
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PRODUCTID:7901
CATALOG#:ARP34122_T100
PRODUCT_NAME:LIG4 (ligase IV, DNA, ATP-dependent) Antibody
PRODUCTD_DESCRIPTION:LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (
UNIT_PRICE:198
QTY:100µg
ACCESSION#:NP_002303
SEARCH_KEYWORD:LIG4,ligase IV, DNA, ATP-dependent,ARP34122_T100,NP_002303,NM_002312,Q8IY66,HGNC:6601
Western_Blot,link:Suggested dilution at 1.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Peptide ID:P05331
Quality_Control,desc:Rabbit IgG purified by protein A chromatography.
ELISA titre using peptide based assay:1:312500
Species Reactivity:Human
Immunoblot,image:ARP34122-QC3450-WB.jpg
Antigen Specificity,desc:Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human LIG4 with an internal ID of P05331
Immunohistochemistry_1,image:ARP34122-QC3450-IHC.jpg
Protein Size,form:911
Key_Reference,desc:Smith,J., et al., (2003) Nucleic Acids Res. 31 (8), 2157-2167
Reconstitution and Storage,desc:Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Nucleotide_Accession#,form,url:NM_002312,
Swissprot_Id,form,url:Q8IY66,
Molecular_Weight,form:104kDa
Gene_Symbol:LIG4
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Also see:
all human DNA ligase IV antibodies
DNA ligase IV antibody review
DNA ligase IV over-expression cell lysate -- positive control for Western blot


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